Journal: Molecular omics
Article Title: Serial In-solution digestion protocol for mass spectrometry-based glycomics and proteomics analysis
doi: 10.1039/d0mo00019a
Figure Lengend Snippet: A, Coverage, total, and unique peptides for standard proteins and proteoglycans as observed by LC-MS/MS analysis after serial in-solution digestion. B, Total proteins for both unique peptides 1 and 2, and coverage of proteoglycan and other ECM related proteins as observed for complex mixtures- mouse brain tissue lysate. ).
Article Snippet: R&D systems) Mouse brain tissue lysate (89-014-501, Abnova) Human neurocan recombinant protein (6508NC050, R&D systems) Aggrecan from bovine articular cartilage (A1960-1MG, SigmaAldrich) Human Syndecan-1 Protein (#7879, BioVision Incorporated) α1-Acid Glycoprotein from human plasma (#G9885, SigmaAldrich) Pierce retention time calibration mixture (#88321, ThermoScientific) Pierce™ HeLa Protein Digest Standard (#88328, Thermo Scientific) Heparan sulfate from porcine intestinal mucosa (HSPIM) (Celsus Laboratories, Inc.0 Heparan sulfate from bovine kidney (HSBK) (SigmaAldrich) 2.2.
Techniques: